Free practice questions

Leaving Cert Biology — Example Quiz

Twenty free Leaving Cert Biology practice questions on cells, enzymes, genetics, ecology and the human body, each with a worked explanation.

20 questions biologyleaving-certsciencesecond-level

Example study questions — not affiliated with, endorsed by, or derived from any examining body's exam papers or question banks.

  1. 1

    Which structure is present in a plant cell but absent from an animal cell?

    • A Mitochondrion
    • B Ribosome
    • C Cellulose cell wall
    • D Nucleus
    Show answer

    Answer: C

    All four structures occur in plant cells, but only the cellulose cell wall is absent from animal cells. Mitochondria, ribosomes and a nucleus are found in both. Note that a cell membrane is present in both — it is the rigid cellulose wall outside the membrane that is unique to plants.

  2. 2

    An enzyme is described as having an optimum pH. What does this mean?

    • A The enzyme can only function at that exact pH and is destroyed at any other
    • B The enzyme works at its fastest rate at that pH
    • C The enzyme changes shape permanently at that pH
    • D The enzyme stops being a catalyst above that pH
    Show answer

    Answer: B

    The optimum is the pH at which the rate of reaction is highest. The enzyme still works either side of it, just more slowly, because the active site shape is progressively distorted. Only at extremes does denaturation become permanent — so B, not A.

  3. 3 Select all that apply

    Which TWO of the following are products of the light stage of photosynthesis? (Select two.)

    • A ATP
    • B Glucose
    • C Oxygen
    • D Carbon dioxide
    Show answer

    Answer: A, C

    The light stage produces ATP, NADPH and oxygen. Oxygen comes from the photolysis (splitting) of water. Glucose is made in the dark stage, using the ATP and NADPH from the light stage. Carbon dioxide is a raw material for the dark stage, not a product.

  4. 4

    Anaerobic respiration in human muscle produces more ATP per glucose molecule than aerobic respiration.

    • A True
    • B False
    Show answer

    Answer: B

    False, and by a wide margin. Aerobic respiration yields far more ATP per glucose because the pyruvate is fully oxidised through Krebs cycle and the electron transport chain. Anaerobic respiration in muscle stops at lactic acid and yields only the small amount of ATP from glycolysis.

  5. 5

    In DNA, adenine always pairs with which base?

    • A Cytosine
    • B Guanine
    • C Thymine
    • D Uracil
    Show answer

    Answer: C

    Adenine pairs with thymine (two hydrogen bonds); cytosine pairs with guanine (three hydrogen bonds). Uracil replaces thymine in RNA, not DNA — a common trap in this question.

  6. 6 Put these in order

    Place the following stages of protein synthesis in the correct order.

    • A mRNA travels from the nucleus to a ribosome
    • B Amino acids are joined by peptide bonds to form a polypeptide
    • C DNA is transcribed into mRNA in the nucleus
    • D tRNA molecules bring amino acids to the ribosome
    Show answer

    Correct order

    1. C DNA is transcribed into mRNA in the nucleus
    2. A mRNA travels from the nucleus to a ribosome
    3. D tRNA molecules bring amino acids to the ribosome
    4. B Amino acids are joined by peptide bonds to form a polypeptide

    Transcription happens first, in the nucleus. The mRNA then leaves through a nuclear pore and attaches to a ribosome. Translation follows: tRNA delivers amino acids matching each codon, and peptide bonds join them into a polypeptide.

  7. 7

    Which blood vessel carries deoxygenated blood?

    • A Pulmonary vein
    • B Pulmonary artery
    • C Aorta
    • D Renal artery
    Show answer

    Answer: B

    The pulmonary artery is the exception to the usual rule: it carries deoxygenated blood from the right ventricle to the lungs. The pulmonary vein is the matching exception, carrying oxygenated blood back to the heart.

  8. 8

    What is the role of bile in digestion?

    • A It digests protein into amino acids
    • B It digests starch into maltose
    • C It emulsifies fats into smaller droplets
    • D It neutralises saliva in the mouth
    Show answer

    Answer: C

    Bile contains no digestive enzymes. It emulsifies fat — breaking large globules into small droplets, which increases the surface area available to lipase. It is produced by the liver, stored in the gall bladder, and released into the small intestine.

  9. 9 Select all that apply

    Which TWO of the following occur during meiosis but not during mitosis? (Select two.)

    • A Chromosomes replicate before division begins
    • B Crossing over occurs between homologous chromosomes
    • C The nuclear membrane breaks down
    • D The chromosome number is halved
    Show answer

    Answer: B, D

    Meiosis is distinguished by crossing over during prophase I and by the halving of chromosome number, producing haploid gametes. Replication before division and breakdown of the nuclear membrane happen in both processes.

  10. 10

    In a food chain, organisms that make their own food are called:

    • A Producers
    • B Primary consumers
    • C Decomposers
    • D Secondary consumers
    Show answer

    Answer: A

    Producers — typically green plants — convert light energy into chemical energy by photosynthesis and form the first trophic level. Consumers obtain energy by eating other organisms; decomposers break down dead material.

  11. 11

    Which vessel in a plant transports water from the roots to the leaves?

    • A Phloem
    • B Stomata
    • C Xylem
    • D Cambium
    Show answer

    Answer: C

    Xylem carries water and dissolved minerals upwards, driven largely by transpiration pull. Phloem transports the products of photosynthesis in both directions. Stomata are pores in the leaf; cambium is a layer of dividing cells.

  12. 12

    Genes carried on the X chromosome are described as sex-linked.

    • A True
    • B False
    Show answer

    Answer: A

    True. Sex linkage refers to genes located on a sex chromosome, in practice usually the X chromosome. Because males have only one X, a single recessive allele is expressed — which is why conditions such as haemophilia and red-green colour blindness are far more common in males.

  13. 13

    A student tests a food sample with Benedict's reagent and heats it. The solution turns brick red. This indicates the presence of:

    • A Starch
    • B Reducing sugar
    • C Protein
    • D Lipid
    Show answer

    Answer: B

    Benedict's reagent turns from blue through green and orange to brick red in the presence of a reducing sugar, the colour reflecting concentration. Starch is tested with iodine (blue-black), protein with the biuret test (purple), and lipid with the brown paper or emulsion test.

  14. 14

    Which organ is chiefly responsible for osmoregulation in humans?

    • A Liver
    • B Pancreas
    • C Lung
    • D Kidney
    Show answer

    Answer: D

    The kidney regulates the water and salt content of the blood, adjusting urine concentration under hormonal control. The liver handles deamination and detoxification, the pancreas secretes enzymes and hormones, and the lungs exchange gases.

  15. 15 Select all that apply

    Which THREE of the following are conditions required for seed germination? (Select three.)

    • A Water
    • B Oxygen
    • C Light
    • D A suitable temperature
    Show answer

    Answer: A, B, D

    Water, oxygen and a suitable temperature are required. Light is not a general requirement — many seeds germinate perfectly well in darkness, and some are inhibited by light. Light becomes essential only afterwards, once the seedling must photosynthesise.

  16. 16

    Two heterozygous tall pea plants (Tt) are crossed. What proportion of the offspring would be expected to be short?

    • A 0%
    • B 25%
    • C 50%
    • D 75%
    Show answer

    Answer: B

    A Tt x Tt cross gives TT, Tt, Tt and tt — a 3:1 ratio of tall to short. Only tt is short, so 25%. The other three carry at least one dominant T allele and are tall.

  17. 17

    What is the function of the ribosome?

    • A Photosynthesis
    • B Storage of genetic material
    • C Protein synthesis
    • D Removal of waste
    Show answer

    Answer: C

    Ribosomes are the site of translation, where mRNA is read and amino acids are assembled into polypeptides. They may be free in the cytoplasm or bound to the rough endoplasmic reticulum.

  18. 18 Put these in order

    Arrange the following levels of ecological organisation from smallest to largest.

    • A Community
    • B Organism
    • C Ecosystem
    • D Population
    Show answer

    Correct order

    1. B Organism
    2. D Population
    3. A Community
    4. C Ecosystem

    An organism is a single individual; a population is all the individuals of one species in an area; a community is all the populations of different species in that area; an ecosystem is the community together with its non-living environment.

  19. 19

    Which of the following best describes a catalyst?

    • A It speeds up a reaction without being used up
    • B It provides the energy needed for a reaction
    • C It is consumed in equal proportion to the reactants
    • D It raises the activation energy of a reaction
    Show answer

    Answer: A

    A catalyst increases the rate of reaction by providing an alternative pathway with lower activation energy, and is unchanged at the end. It does not supply energy, and it is not consumed — which is why enzymes work in very small quantities.

  20. 20

    Arteries carry blood towards the heart and veins carry blood away from it.

    • A True
    • B False
    Show answer

    Answer: B

    False — it is the other way round. Arteries carry blood away from the heart, which is why they have thick muscular walls to withstand high pressure. Veins return blood to the heart at lower pressure and contain valves to prevent backflow.


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